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在营养液中加入不同浓度硅且接种黄瓜霜霉病菌后,通过调查其病情指数和检测黄瓜叶片内硅元素含量及过氧化物酶(guaiacol-peroxidase,POD)、多酚氧化酶(polyphenol oxidase,PPO)、苯丙氨酸解氨酶(phenylalanine ammonia-lysae,PAL)、β-1,3葡聚糖酶(β-1,3-glucanase)、超氧化物歧化酶(superoxidedismutase,SOD)5种抗霜霉病相关酶活性,探讨硅抑制黄瓜霜霉病的生理生化机制。结果表明,营养液中硅浓度为100mg/L的处理,黄瓜霜霉病病情指数为21.3,防治效果达到62.8%;营养液中硅浓度与黄瓜叶片内硅元素含量呈正相关,200mg/L处理叶片内硅元素含量最高,且7天后达到2.98mg/g;加硅处理接种黄瓜霜霉病菌后,黄瓜叶片抗病相关酶活性变化明显且差异达显著水平,其中硅浓度为100～200mg/L时上述5种酶活性最高。
After adding different concentrations of silicon into the nutrient solution and inoculating the cucumber downy mildew, the disease index and the content of silicon in leaves of cucumber and the content of peroxidase (POD) and polyphenol oxidase (PPO), phenylalanine ammonia-lysae (PAL), β-1,3-glucanase and superoxide dismutase (SOD) Downy mildew-related enzyme activity to explore the physiological and biochemical mechanisms of silicon inhibition of cucumber downy mildew. The results showed that the disease index of Cucumber downy mildew was 21.3 and the control effect was 62.8% when the concentration of silicon was 100mg / L in nutrient solution. The concentration of silicon in nutrient solution was positively correlated with the content of silicon in cucumber leaf. The contents of silicon in the leaves were the highest, reaching 2.98 mg / g after 7 days. After adding silicon to the cucumber downy mildew pathogen, the activities of enzymes related to disease resistance of the cucumber leaves changed significantly and the differences reached significant level. When the silicon concentration was 100 ~ 200 mg / L The above five kinds of enzyme activity of the highest.