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按照第11届国际组织相容性抗原研讨会（IHW）工作会议HLAⅡ类PCR－SSO分型标准和美国国立骨髓供者计划组织（NMDP）对HLADRB基因分型要求，设计合成一对引物，可同时扩增HLA-DRB1＋B3＋B4＋BSDNA片段，长度为256bp，设计合成不同片段大小探针27种，可检出DRB座位上DRB1的39种等位基因，DRB3的3种等位基因，DRB4的1种等位基因和DRB5的3种等位基因。
According to the HLA Class II PCR-SSO typing criteria and IHDP genotyping requirements of the 11th International Organization for the Study of Compatible Antigen (IHW) Working Conference and the National Plan of the United Kingdom National Marrow Donor Program (NMDP), we designed and synthesized a pair of primers At the same time amplifying HLA-DRB1 + B3 + B4 + BSDNA fragment with a length of 256bp, 27 probes of different fragment sizes were designed and synthesized, which could detect 39 alleles of DRB1, 3 alleles of DRB3, 1 allele of DRB4 3 alleles of DRB5 and genes.