Objective:To investigate the correlation between the insulin gene variable number tandem repeats(INS-VNTR) with polycystic ovary syndrome(PCOS) and metabolic features related to insulin resistance(IR). Methods:One hundred and thirty patients with PCOS(PCOS group) and 130 normal women(control group) were included.Genotyping of INS-VNTR was performed using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). Results:The distribution of genotype of INS-VNTR was similar in PCOS group,but theⅢallele frequency of INS-VNTR was higher in PCOS patients than that in controls,and Logistic regression analysis revealed that theⅢallele was associated with increased risk of PCOS[adjusted odd ratio(OR) =2.31;95%confidence interval(CI) = 1.07-4.98],compared with theⅠallele.The distribution of genotype and theⅢallele frequency of INS-VNTR in PCOS insulin resistance(PCOS-IR) group were significantly higher than that in PCOS non-insulin resistance group (PCOS-NIR).Moreover,PCOS women withⅢallele had statistically significantly higher fasting insulin level and HOMA-IR than those of PCOS women withⅠallele. Conclusion:INS-VNTR may not be a susceptibility gene,but INS-VNTR polymorphism may play an important role in the occurrence of insulin resistance in patients with PCOS. Objective: To investigate the correlation between the insulin gene variable number tandem repeats (INS-VNTR) with polycystic ovary syndrome (PCOS) and metabolic features related to insulin resistance (IR). Methods: One hundred and thirty patients with PCOS (PCOS group) and 130 normal women (control group) were included. Genotyping of INS-VNTR was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: The distribution of genotype of INS-VNTR was similar in PCOS group, but theⅢallele frequency of INS-VNTR was higher in PCOS patients than that in controls, and Logistic regression analysis revealed that theⅢallele was associated with increased risk of PCOS [adjusted odd ratio (OR) = 2.31; 95% confidence interval (CI) = 1.07- 4.98], compared with the Iallele. The distribution of genotype and the IIIallele frequency of INS-VNTR in PCOS insulin resistance (PCOS-IR) group were significantly higher than that in PCOS non-insulin resistance group (PCOS-NIR) , PCOS women withⅢallele had statistically significantly higher fasting insulin level and HOMA-IR than those of PCOS women withⅠallele. Conclusion: INS-VNTR may not be a susceptibility gene, but INS-VNTR polymorphism may play an important role in the occurrence of insulin resistance in patients with PCOS.