论文部分内容阅读
目的:探讨siRNA靶向人c-myc基因对人喉鳞状细胞癌Hep-2细胞生长增殖的抑制作用。方法:根据人c-myc基因设计和合成siRNA和随机的siN.C作为阴性对照,通过转染载体Lipofectamine2000脂质体转染Hep-2细胞,进行MTT、形态学和实时PCR法检测,观察其干扰效果。结果:①MTT结果显示人端粒酶siRNA能有效抑制Hep-2细胞增殖;②实时PCR结果显示转染组与阴性对照组和空白对照组相比,c-mycmR-NA表达明显减弱,转染组S3c-mycmRNA抑制率达到94%;③形态学结果显示人c-mycsiRNA能有效抑制Hep-2细胞增殖,肿瘤细胞异形性减小。结论:靶向c-myc的siRNA能显著抑制喉鳞状细胞癌生长增殖。
Objective: To investigate the inhibitory effect of siRNA targeting human c-myc gene on human laryngeal squamous cell carcinoma Hep-2 cell proliferation. Methods: siRNA was designed and synthesized based on human c-myc gene and randomized siRNA was used as negative control. Hep-2 cells were transfected with lipofectamine 2000 by MTT, morphology and real-time PCR Interference effect. Results: ① MTT results showed that human telomerase siRNA can effectively inhibit the proliferation of Hep-2 cells; ②The real-time PCR results showed that the expression of c-mycmR-NA was significantly decreased in the transfected group compared with the negative control group and the blank control group. The inhibitory rate of S3c-mycmRNA reached 94%. ③The morphological results showed that human c-mycsiRNA could effectively inhibit the proliferation of Hep-2 cells and reduce the atypia of tumor cells. Conclusion: siRNA targeting c-myc can significantly inhibit the growth of laryngeal squamous cell carcinoma.