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目的研究血小板α粒子蛋白包括血小板反应素1(thrombospondin1,TSP1),血小板因子4(plateletfactor4,PF4),β血小板球蛋白(βThromboglobulin,βTG)和血小板源性生长因子(plateletderivedgrowthfactor,PDGF)对巨核细胞生成的影响和可能的作用机制。方法用血浆凝块巨核细胞集落培养,观察对生长的影响,并进一步使用免疫组化和Westernblot证实受体的存在和对cfos表达的影响。结果(1)TSP1和PF4显著地抑制巨核细胞集落(CFUMK)的形成,有意义的起始抑制浓度为25μg/ml(P<005)。比较相同浓度的作用TSP1和PF4有相似的抑制效应,但βTG则无显著的影响。而PDGF显著地刺激巨核细胞的生成,最大的刺激剂量为50ng/ml;(2)血小板生长因子(TPO)(20ng/ml)显著地增加CFUMK的形成,但当加上TSP1(5μg/ml)时。TPO对巨核细胞的生长作用能被TSP1减弱(71±55/2×105细胞~41±80/2×105细胞,P<001);(3)证实骨髓的巨核细胞表达TSP受体(C?
Objective To study the expression of platelet α-particle protein including thrombospondin 1 (TSP1), platelet factor 4 (PF4), βThromboglobulin (βTG) and plateletderived derived growth factor, PDGF) on megakaryocytopoiesis and its possible mechanism. Methods The coagulation of megakaryocytes in plasma clot was used to observe the effects on the growth of the cells. Immunohistochemistry and Western blot were used to confirm the existence of receptor and the effect on c-fos expression. Results (1) TSP1 and PF4 significantly inhibited the formation of megakaryocyte colonies (CFU MK), a significant initial inhibitory concentration of 2 5μg / ml (P <0 05). Compared with the same concentration of TSP1 and PF4 have similar inhibitory effect, but β TG had no significant effect. While PDGF significantly stimulated the production of megakaryocytes, the maximum stimulation dose was 50ng / ml; (2) TPO (20ng / ml) significantly increased the CFU-MK formation, ml). The effect of TPO on the growth of megakaryocytes was attenuated by TSP1 (71 ± 55 / 2 × 105 cells ~ 41 ± 80 × 2 × 105 cells, P <001). (3) The expression of megakaryocyte in bone marrow was confirmed TSP receptor (C?