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目的:观察六月青总皂苷(TLYQ)的体外抗乙型肝炎病毒的作用。方法:采用HepG2.2.15细胞模型进行体外培养,给予不同浓度TLYQ,以拉米夫定作阳性对照,作用72h后检测上清液中HBsAg、HBeAg的分泌,观察药物对HepG2.2.15细胞分泌HBV病毒抗原的影响,同时以四甲基偶氮唑盐(MTT)比色法检测药物在体外对HepG2.2.15细胞的生长抑制作用,从而评价药物的抗HBV作用。结果:TLYQ对HepG2.2.15细胞的50%生长抑制率(TC50)为349.51mg·L-1,对HepG2.2.15细胞分泌HBsAg的50%抑制率(IC50)为9.45mg·L-1,其治疗指数(TI)为36.98。结论:TLYQ在体外有显著的抗HBV的作用,且毒性较低。
Objective: To observe the anti-hepatitis B virus effect of total saponin (TLYQ) in vitro. METHODS: HepG2.2.15 cell model was used for in vitro culture, different concentrations of TLYQ were given, lamivudine was used as a positive control, and the secretion of HBsAg and HBeAg was detected in the supernatant after 72 hours. The drug was observed to secrete HBV virus in HepG2.2.15 cells. The effect of the antigen was also tested by the MTT colorimetric assay to determine the growth inhibitory effect of the drug on HepG2.2.15 cells in vitro, thereby evaluating the anti-HBV effect of the drug. RESULTS: The 50% growth inhibition rate (TC50) of TLYQ on HepG2.2.15 cells was 349.51 mg·L-1, and the 50% inhibition (IC50) of HBsAg secretion on HepG2.2.15 cells was 9.45 mg·L-1. The index (TI) is 36.98. Conclusion: TLYQ has significant anti-HBV effect in vitro and has low toxicity.