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目的探讨补肾化痰方对多囊卵巢综合征(PCOS)大鼠胰岛素抵抗的影响。方法采用皮下注射脱氢表雄酮(DHEA)造模法建立PCOS大鼠模型,将大鼠随机分为正常对照组、模型对照组、二甲双胍组、中药(补肾化痰方)低剂量组、中药(补肾化痰方)高剂量组,给予相应药物连续灌胃28天。行阴道涂片、HE染色观察动情周期;用酶联免疫吸附测定法(ELISA)检测血清胰岛素(INS)水平;用电子精密天平测量脂肪湿重;用血糖仪测定大鼠血糖水平,计算葡萄糖曲线下面积(AUC)、胰岛素抵抗指数(HOMA-IR)及胰岛素敏感指数(ISI)。结果与对照组相比,模型组大鼠空腹血糖、空腹胰岛素、葡萄糖曲线下面积升高,HOMA指数增高,ISI降低,无规律的动情周期,无排卵,体内脂肪增加。二甲双胍和补肾化痰方两个剂量组可不同程度的改变PCOS大鼠上述病理改变,补肾化痰方用药剂量较高效果较好。结论补肾化痰方可改善多囊卵巢综合征大鼠高胰岛素血症、胰岛素抵抗状态,降低体脂水平,恢复大鼠动情周期及排卵。
Objective To investigate the effect of Bushen Huatan Fang on insulin resistance in rats with polycystic ovary syndrome (PCOS). Methods PCOS rat model was established by subcutaneous injection of DHEA model. The rats were randomly divided into normal control group, model control group, metformin group, traditional Chinese medicine (Bushen Huatan Fang) low dose group, (Bushen Huatan Fang) high-dose group, given the corresponding drug for 28 days. The vaginal smears were taken and the estrous cycle was observed by HE staining. The level of serum insulin (INS) was measured by enzyme-linked immunosorbent assay (ELISA). The wet weight of fat was measured by electronic balance. The blood glucose level was measured by glucose meter. Area under the curve (AUC), insulin resistance index (HOMA-IR) and insulin sensitivity index (ISI). Results Compared with the control group, the area under fasting blood glucose, fasting insulin and glucose in model group increased, HOMA index increased, ISI decreased, irregular estrous cycle, anovulation and increased body fat. Metformin and Bushen Huatan Fang two dose groups can change the above pathological changes of PCOS rats to varying degrees, Bushen Huatan Fang higher dose of better results. Conclusion Bushen Huatan Fang can improve hyperinsulinemia and insulin resistance in polycystic ovary syndrome rats, decrease body fat level and restore estrous cycle and ovulation in rats.