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目的对内皮祖细胞(endothelial progenitor cells,EPCs)源性胞外囊泡(extracellular vesicles,EVs)(EPC-EVs)的分离纯化、生物学特性和功能方面的相关研究进行综述。方法广泛查阅近年来国内外EPC-EVs相关文献,进行综合分析和归纳总结。结果目前用于分离纯化EPC-EVs的方法主要为差速离心法。EPC-EVs在透射电镜下为形态较均一的球形颗粒,直径多为60~160 nm。EPC-EVs表达其来源细胞EPCs的表面标志物,如CD31、CD34和CD133;不表达血小板相关标志物P-选择素和CD42b以及单核细胞标志物CD14。研究发现,EPC-EVs具有对缺血性损伤、抗Thy-1肾炎和心肌肥厚的修复功能以及对心脑血管疾病的预测价值。结论 EPCEVs的研究前景广阔,但其所含成分、生物学功能和作用机制等还有待深入研究。
OBJECTIVE: To study the isolation, purification, biological characteristics and function of endothelial progenitor cells (EPCs) derived extracellular vesicles (EVs). Methods The references of EPC-EVs at home and abroad in recent years are consulted extensively and analyzed comprehensively and summarized. Results Currently, the method used for isolation and purification of EPC-EVs is differential centrifugation. Under the TEM, EPC-EVs are spherical particles with a diameter of 60 ~ 160 nm. EPC-EVs expressed surface markers of EPCs derived from their cells, such as CD31, CD34 and CD133; did not express P-selectin and CD42b, a marker of platelet associated with monocyte marker CD14. The study found that, EPC-EVs has the damage of ischemic injury, anti-Thy-1 nephritis and myocardial hypertrophy repair function and the predictive value of cardiovascular and cerebrovascular diseases. Conclusions The research of EPCEVs is promising, but the composition, biological function and mechanism of EPCEVs still need further study.