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TK基因一GCV系统是最有希望在临床上用于肿瘤基因治疗的方法之一。为了提高基因靶向表达的效率，使之可以应用于体内实验，我们构建重组腺病毒AdCMVTK和带有CMV、CEA两个启动子的AdCMVCEATK，在五种癌细胞中进行了体外实验。发现腺病毒介导的TK基因赋予癌细胞GCV敏感性比对照组高2～3个数量级，并使感染病毒和未感染病毒细胞比例为110时仍产生较强的“旁观效应”。实验表明PG肺癌，CAn结肠癌，HeLa细胞是腺病毒介导的TK基因的理想靶细胞。
The TK gene-GCV system is one of the most promising methods for the clinical use of tumor gene therapy. In order to increase the efficiency of gene-targeted expression and make it applicable to in vivo experiments, we constructed recombinant adenovirus AdCMVTK and AdCMVCEATK with two promoters of CMV and CEA, and performed in vitro experiments in five cancer cells. It was found that adenovirus-mediated TK gene confers GCV sensitivity to cancer cells by 2 to 3 orders of magnitude higher than that of the control group, and still produces a strong “bystander effect” when the proportion of infected and uninfected virus cells is 110. Experiments have shown that PG lung cancer, CAn colon cancer, and HeLa cells are ideal adenovirus-mediated TK gene target cells.