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目的探讨可溶性B7-H3(sB7-H3)在脓毒血症患者体内的表达特征及其生物学意义。方法通过ELISA方法检测脓毒血症患者体内sB7-H3及其他炎症因子的表达水平,并对相关性进行分析。利用分离纯化的单核细胞,分析肿瘤坏死因子-α(TNF-α)在sB7-H3产生中的调控作用。利用B7-H3Ig融合蛋白,体外实验分析sB7-H3对单核细胞的生物学作用。结果脓毒血症患者与健康对照组相比,血清sB7-H3水平显著升高(P<0.01)。sB7-H3表达水平与TNF-α及基质金属蛋白酶-9(MMP-9)成显著正相关(分别P<0.01和<0.05)。TNF-α调控sB7-H3的产生,同时sB7-H3又可作用于单核细胞,促进TNF-α分泌表达(均P<0.01)。结论脓毒血症患者体内存在高水平sB7-H3,其水平受TNF-α调控,功能则表现为刺激单核细胞分泌TNF-α。
Objective To investigate the expression of soluble B7-H3 (sB7-H3) in sepsis patients and its biological significance. Methods The expression levels of sB7-H3 and other inflammatory factors in sepsis patients were detected by ELISA, and the correlation was analyzed. The purified monocytes were used to analyze the regulatory effect of tumor necrosis factor-α (TNF-α) on sB7-H3 production. Using B7-H3Ig fusion protein, the biological effects of sB7-H3 on monocytes were analyzed in vitro. Results Serum sB7-H3 levels were significantly higher in patients with sepsis compared with healthy controls (P <0.01). The expression of sB7-H3 was positively correlated with TNF-α and MMP-9 (P <0.01 and <0.05, respectively). TNF-α regulates the production of sB7-H3, while sB7-H3 can act on monocytes and promote the secretion of TNF-α (all P <0.01). Conclusions There is high level of sB7-H3 in sepsis patients. The level of TNF-α is regulated by TNF-α, while TNF-α is stimulated by monocytes.