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目的：建立血中华法林浓度的毛细管胶束电动色谱分析方法，并对其进行评价。方法：用二氯甲烷提取血清中华法林，５－乙基－５－Ｐ－甲苯基巴比妥酸为内标，操作电压１５ｋＶ，２１０ｎｍ波长检测。电泳缓冲液由２５ｍｍｏｌ／Ｌ硼酸盐，２５ｍｍｏｌ／Ｌ十二烷基硫酸钠，１０ｍｍｏｌ／Ｌ氯化钠（ｐＨ９．０）组成。结果：日内ＲＳＤ１．４％～６．２％，日间ＲＳＤ１．４％～４．７％，在０～１０μｇ／ｍｌ范围内呈线性，最低血清华法林检测浓度为０．０３μｇ／ｍｌ。测定结果与反相高效液相色谱法进行了比较，两种方法有很好相关性，ｒ＝０．９９６７（Ｐ＞０．１）。结论：毛细管胶束电动色谱法精密度、准确度高，专一性强，是一种较理想的血药浓度检测方法。
OBJECTIVE: To establish capillary micellar electrokinetic chromatographic method for the determination of warfarin concentration in blood. Methods: Warfarin was extracted from the serum with dichloromethane, and 5-ethyl-5-p-tolylbarbituric acid was used as the internal standard. The operating voltage was detected at 15 kV and 210 nm. The electrophoresis buffer consists of 25 mmol / L borate, 25 mmol / L sodium lauryl sulfate, 10 mmol / L sodium chloride (pH 9.0). Results: Intraday RSD ranged from 1.4% to 6.2% and daytime RSD ranged from 0.4% to 4.7%. The linear range was from 0 to 10 μg / ml. The lowest serum warfarin concentration was 0.03 μg / ml. The results were compared with reversed-phase high-performance liquid chromatography, the two methods have a good correlation, r = 0.9967 (P> 0.1). Conclusion: Capillary micellar electrokinetic chromatography is more accurate, accurate and specific, and it is an ideal method for the determination of plasma concentration.