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目的从珊瑚共生真菌中获得抗肿瘤药物先导化合物。方法通过集成化学与细胞毒性的筛选方法筛选目标菌株,采用现代色谱和波谱的方法分离鉴定目标菌株的活性代谢产物,通过化学沟通鉴定化合物的绝对立体构型。结果和结论从采自广西涠洲岛的花刺柳珊瑚(Echinogorgia floraNutting)中筛选获得了1株具有细胞毒活性的青霉菌(Penicillium sp.)gxwz406,从其发酵产物中分离鉴定了5个化合物:S(-)-2-(2-hydroxypropanamido)benzamide(1)、R(+)-chrysogine(2)2、-pyruvylaminobenzamide(3)、nicotinamide(4)和2’,3’-dihydrosorbicillin(5),并首次确定了化合物1的绝对构型。
Objective To obtain antitumor drug lead compounds from coral symbiotic fungi. Methods The target strains were screened by integrated chemistry and cytotoxicity screening methods. The active metabolites of the target strains were isolated and identified by modern chromatography and spectroscopic methods. The absolute stereochemistry of the compounds was identified by chemical communication. RESULTS AND CONCLUSIONS One of the cytotoxic Penicillium sp. Gxwz406 strains was screened from Echinogorgia floraNutting collected from Weizhou Island, Guangxi. Five compounds were isolated and identified from their fermentation products (2) 2 -pyruvylaminobenzamide (3), nicotinamide (4) and 2 ’, 3’-dihydrosorbicillin (5) , And for the first time confirmed the absolute configuration of compound 1.