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Objective In order to establish tumour cell line and screen anticancer drugs. Methods We froze cell line in liquid nitrogen container with 10% glycerin. Some of them were kept more than 15 years. Results Cell livability rate arrived at 80%～90% after anabiosis. Conclusion Our cell store is suitable to offer cell line for scientific research and for complete task of investigation.
Subject We froze cell line in liquid nitrogen container with 10% glycerin. Some of them were kept more than 15 years. Results Cell livability rate arrived at 80%～90% after anabiosis . Conclusion Our cell store is suitable to offer cell line for scientific research and for complete task of investigation.