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【目的】利用培养法从日本三宅岛火山土壤(堆积年限131年)中分离到一株能氧化分解硫代硫酸盐的细菌MU2A-22T。【方法】用培养法对该菌株MU2A-22T进行了生理生化性质以及分类学位置上的确定。【结果】菌株MU2A-22T为革兰氏阴性,短杆状或球状。理化性质表明该菌株能利用葡萄糖、L-阿拉伯糖、葡萄糖酸盐、己二酸酯、dL-苹果酸钠、硫代硫酸钠(最适浓度为2.5 mmol/L)为唯一碳源进行自养生长。最适生长温度为25°C 30°C,最适pH为6.0 8.0。菌株MU2A-22T的16S rRNA序列与菌株Paracoccus solventivorans 6637T亲缘关系最近,序列相似性为97%,编码核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubisco)的基因也被确定。对Paracoccus属内几种近缘菌的脂肪酸分析,证明菌株MU2A-22T中含有Paracoccus属的特征氨基酸,其中含量大于10%的分别为C18:1(74.7%)和C18:0(12.1%)。DNA-DNA杂交实验表明,菌株MU2A-22T与Paracoccus solventivorans 6637TDNA的相似度为49.3%。MU2A-22T菌株G+C含量为66.5%66.7%。【结论】菌株MU2A-22T为Paracoccus属内的一新种菌(登录号GQ452286),命名为Paracoccus scorialis sp.nov.。
【Objective】 A bacterial strain MU2A-22T capable of oxidizing and decomposing thiosulfate was isolated from the volcanic soil of Miyakejima (131 years of accumulation) in Japan by culture method. 【Method】 The physiological and biochemical characteristics and taxonomic position of this strain MU2A-22T were determined by culture method. 【Result】 The strain MU2A-22T was Gram-negative, short rod-shaped or globular. The physicochemical properties indicated that the strain could utilize self-recuperation of glucose, L-arabinose, gluconate, adipate, sodium dL-malate and sodium thiosulfate (the optimum concentration was 2.5 mmol / L) long. The optimum growth temperature is 25 ° C 30 ° C, the optimum pH is 6.0 8.0. The 16S rRNA sequence of strain MU2A-22T was the closest to the strain Paracoccus solventivorans 6637T with a sequence similarity of 97% and the gene encoding ribulose-1,5-bisphosphate carboxylase / oxygenase (Rubisco) was also identified . Fatty acid analysis of several species of Paracoccus showed that strain MU2A-22T contained the characteristic amino acids of Paracoccus sp., With C18: 1 (74.7%) and C18: 0 (12.1%), respectively. DNA-DNA hybridization experiments showed that the similarity between strain MU2A-22T and Paracoccus solventivorans 6637TDNA was 49.3%. The G + C content of MU2A-22T strain was 66.5% and 66.7%. 【Conclusion】 The strain MU2A-22T is a new species in the genus Paracoccus (accession number GQ452286), named Paracoccus scorialis sp. Nov.