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口腔上皮粘膜细胞是学生所必须观察的材料。以水为介质制做的临时装片,只能观察到口腔上皮粘膜细胞的平面图形。经大量的实验研究,我配出一种混合介质以代替水制作临时染色装片,即可在一般显微镜下,观察到口腔上皮粘膜细胞的立体形状及其内部结构,这对学生进一步理解细胞的立体形状以及动植物细胞在结构上的不同,是大有帮助的。将方法介绍如下: 取丙三醇(甘油,分析纯)和95%乙醇各一份,预先混合成甘油酒精溶液待用。在室温下,制成蔗糖的饱和水溶液。实验时,取蔗糖饱和水溶液一份(用量和甘油或酒精量相同),加入到预先配好的甘油酒精混合液中,配成1:1:1甘油、酒精、蔗糖饱和液的混合介质(以下简
Oral epithelial mucosal cells are the material students must observe. Water as a medium made of temporary loading film, only observed oral mucosal cells in the plane pattern. After a large number of experimental studies, I drew a mixed medium instead of water to make a temporary dyed piece, you can observe the oral epithelial mucosal cells under normal microscope three-dimensional shape and its internal structure, which students further understand the cell Three-dimensional shape and plant and animal cells in the structural differences, is very helpful. The method described below: Take glycerol (glycerol, analytical grade) and 95% ethanol each one, pre-mixed into glycerol alcohol solution stand-by. A saturated aqueous sucrose solution was prepared at room temperature. The experiment, take a saturated sucrose solution (amount and the same amount of glycerol or alcohol), added to the pre-mixed glycerol alcohol mixture, dubbed 1: 1: 1 glycerol, alcohol, sucrose saturated medium (below simple