根据甜瓜黄斑病毒(Melon yellow spot virus,MYSV)N基因的保守序列设计3组特异引物对,通过一系列优化,筛选并获得1组特异引物,建立了MYSV的RT-LAMP检测方法。该方法可在62~64℃,45 min内完成对样品的检测,并且能够特异性扩增MYSV,灵敏度为RT-PCR的100倍,特异性强,灵敏度高,适合对MYSV病样快速检测与鉴定。 Three sets of specific primers were designed according to the conserved sequence of N gene of Melon yellow spot virus (MYSV). RT-LAMP assay was established for MYSV by a series of optimization, screening and obtaining a set of specific primers. The method can detect samples at 62-64 ℃ in 45 min, and can specifically amplify MYSV with a sensitivity of 100-fold that of RT-PCR. The method has the advantages of high specificity and high sensitivity, and is suitable for rapid detection of MYSV-like diseases Identification.