目的:观察前列腺素E2受体亚型3(EP3)激动剂ONO-AE-248对中性粒细胞的影响。方法:运用电子显微镜、MTS法、RT-PCR和流式细胞术等技术,对中性粒细胞的形态变化、生物学活性及凋亡进行观察和评估,并对EP3受体进行分析。结果:中性粒细胞存在EP3受体基因的表达,以5mmol/L的ONO-AE-248作用12h后,其多叶核融合为单叶核,核膜起泡、破裂,核质溢出,细胞死亡;但其快速死亡不具有细胞凋亡和坏死的形态特征且需要能量(ATP)供应。结论:ONO-AE-248所致这种新型细胞主动死亡的形式,可能为“非凋亡性程序化细胞死亡”的一种,这对于重新认识和定位中性粒细胞在非特异性免疫中的作用和地位提出了新的思路。 Objective: To investigate the effect of ONO-AE-248, a prostaglandin E2 receptor subtype 3 (EP3) agonist, on neutrophils. Methods: Morphological changes, biological activity and apoptosis of neutrophils were observed and evaluated by electron microscopy, MTS, RT-PCR and flow cytometry. EP3 receptor was analyzed. Results: The expression of EP3 receptor gene existed in neutrophils. After treated with 5mmol / L ONO-AE-248 for 12h, the multilobate nucleus fused to a single lobe nucleus. The nuclear membrane blistered and ruptured, But its rapid death does not have the morphological features of apoptosis and necrosis and requires energy (ATP) supply. CONCLUSIONS: This novel form of cell death by ONO-AE-248 may be a form of “non-apoptotic programmed cell death,” which is important for re-understanding and locating neutrophils in nonspecific immunity Role and status proposed a new way of thinking.