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本实验在建立ＤＮＡ多聚酶α活性测定方法的基础上观察了商陆多糖Ｉ（ＰＡＰ－Ｉ）对小鼠淋巴细胞增殖及淋巴细胞ＤＮＡ多聚酶α活性的影响。采用［３Ｈ］－ＴｄＲ掺入法和模板活化法（［３Ｈ］－ｄＴＴＰ掺入法）分别检测ＰＡＰ－Ｉ给药组及对照组小鼠淋巴细胞增殖能力和淋巴细胞ＤＮＡ多聚酶α活性。体外实验发现ＰＡＰ－Ｉ显著增强ＣｏｎＡ诱导的小鼠脾淋巴细胞增殖及其ＤＮＡ多聚酶α的活性；小鼠腹腔注射ＰＡＰ－Ｉ（每周１次）对脾淋巴细胞ＤＮＡ多聚酶α活性基本上无影响，但加入ＣｏｎＡ（５μｇ／ｍｌ）刺激后，ＰＡＰ－Ｉ１０ｍｇ／ｋｇ剂量组ＤＮＡ多聚酶α活性显著增强。结果提示ＰＡＰ－Ｉ增强ＤＮＡ多聚酶α活性可能是促进脾淋巴细胞增殖、增强免疫功能的机制之一。
In this experiment, based on the establishment of DNA polymerase α activity assay method, the effects of PAP-I on lymphocyte proliferation and lymphocyte DNA polymerase α activity in mice were observed. The [3H]-TdR incorporation method and the template activation method ([3H]-dTTP incorporation method) were used to detect the lymphocyte proliferation and lymphocyte DNA polymerase alpha activity in PAP-I-treated and control mice. In vitro experiments found that PAP-I significantly enhanced ConA-induced mouse spleen lymphocyte proliferation and DNA polymerase alpha activity; mice injected intraperitoneally with PAP-I (once per week) had no effect on spleen lymphocyte DNA polymerase alpha activity However, after stimulation with ConA (5 μg/ml), the activity of DNA polymerase α was significantly increased in the PAP-I 10 mg/kg dose group. The results suggest that PAP-I enhances DNA polymerase alpha activity may be one of the mechanisms that promote spleen lymphocyte proliferation and enhance immune function.