报道了水蛭素全基因的合成及克隆。根据已知水蛭素氨基酸序列及酵母菌体内蛋白质表达特性 ,设计了水蛭素的全基因序列 ,采用化学合成及酶学相结合的方法 ,合成了该基因片段 ,并逐段克隆至载体 pBS上 ,得到全长水蛭素基因。序列测定表明 ,克隆后DNA顺序同原设计序列。 Reported the synthesis of hirudin gene and cloning. According to the known hirudin amino acid sequence and the protein expression characteristics of yeast in vivo, the whole gene sequence of hirudin was designed. The gene fragment was synthesized by a combination of chemical synthesis and enzymology, and cloned into vector pBS, Get full-length hirudin gene. Sequence analysis showed that the cloned DNA sequence with the original design sequence.