生物分子固定化或传感界面设计技术是研制压电免疫传感器的关键之一 .本文结合自组装单分子膜 (SAMs)和聚电解质静电吸附组装技术 ,提出了一种新的压电免疫传感器中生物分子固定化方法 ,研制成一种检测补体C3的压电免疫传感器 .先在石英晶振的金电极表面组装一层胱胺SAMs,再在膜上组装带相反电荷的聚苯磺酸钠 (PSS)单层膜 ,通过静电吸附作用固定抗体 (抗原 ) ,实现对相应抗原 (抗体 )的检测 .利用扫描电镜技术 ,从形态上考察了晶振组装胱氨SAMs与PSS及固定补体C3 抗体后的表面形貌 .研究了抗体的固定化条件 ,探讨了传感器采用这种固定化方法的响应与再生性能 ,并与戊二醛键合固定法进行比较 .结果表明 ,这种固定化方法不仅对蛋白质类生物分子的固定化具有普适性 ,而且对所固定的生物分子的活性影响小 ,传感器响应的频移值大 ,灵敏度高 ,选择性和再生性能均较好 Biomolecule immobilization or sensing interface design technique is one of the key points in the development of piezoelectric immunosensor.In this paper, a novel piezoelectric immunosensor is proposed based on self-assembly monolayer (SAMs) and polyelectrolyte electrostatic adsorption assembly Biomolecule immobilization method, a kind of piezoelectric immunosensor for detecting complement C3 was developed.A layer of cystamine SAMs was first assembled on the surface of gold electrode of quartz crystal, and the oppositely charged sodium polybenzene sulfonate (PSS) Monolayer, immobilize the antibody (antigen) by electrostatic adsorption to detect the corresponding antigen (antibody). Using scanning electron microscopy, the surface morphology of the assembled cystamine SAMs and PSS and the fixed complement C3 antibody The immobilization condition of the antibody was studied, the response and regeneration performance of the immobilized sensor were discussed, and the comparison with the glutaraldehyde bonded immobilization method was carried out.The results showed that this immobilization method was not only effective for proteomics Immobilization of molecules is universal, but also has little effect on the activity of the immobilized biomolecules. The frequency response value of the sensor response is large, the sensitivity is high, the selectivity is Student performance are better