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本文作者用羟胺和巯基乙醇处理乙型流感病毒血凝素(HA)和丙型流感病毒糖蛋白(HEF),发现此两种糖蛋白分别通过不稳定的硫脂型键,与十六烷酸和硬脂酸酰化。许多病毒和细胞蛋白质多肽骨架上共价附着脂防酸。实验中用~3H葡糖胺作放射标记,用~(14)C氨基酸作标记平行对照,在有~3H标记的十六烷酸存在下,乙型和丙型流感病毒分别在CEF和MDCK细胞中生长。在凝胶放射图上表明乙型和丙型流感病毒血凝素糖蛋白
The authors used hydroxylamine and mercaptoethanol treatment of influenza virus hemagglutinin (HA) and influenza virus glycoprotein (HEF), found that the two glycoproteins were unstable sulfur-type bonds, and hexadecanoic acid And stearic acid acylation. Many viral and cellular protein polypeptide covalently attached to the lipid skeleton anti-acid. In the experiment, ~ 3H glucosamine was used as a radiolabel and the ~ (14) C amino acid was used as a parallel control. In the presence of ~ 3H labeled hexadecanoic acid, influenza B and C viruses were respectively expressed in CEF and MDCK cells Growing. The hemagglutinin glycoproteins of influenza B and C viruses are indicated on gel radiographs