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为筛选出在苗期和孕穗期都具有强耐冷性的种质以供水稻耐冷研究与育种利用,本研究利用人工低温胁迫的方法,对20份苗期耐冷的普通野生稻染色体片段代换系进行了孕穗期耐冷性鉴定评价。结果表明:(1)在20份代换系材料中,孕穗期耐冷性表现强(3级)、中(5级)和弱(7级)级别的材料为2份、15份和3份,分别占供试材料的10%、75%和15%;(2)水稻苗期和孕穗期的耐冷性呈显著正相关(p<0.05),所鉴定出的2份抗性3级材料(DC907和DC866)不但可以作为苗期耐冷性抗源,同时也可以作为孕穗期耐寒性抗源;(3)可溶性蛋白、可溶性糖、游离氨基酸、脯氨酸、丙二醛和超氧化物歧化酶等各项生理指标与相对结实率之间均无显著的相关关系,因此均不适宜作为孕穗期耐冷性的鉴定评价指标。
In order to screen out germplasm with strong cold tolerance at seedling and booting stages for cold tolerance and breeding use of rice, this study used artificial low temperature stress method to screen 20 cold-tolerant common wild rice chromosome segments substitution lines The booting cold tolerance identification evaluation. The results showed that: (1) Cold tolerance at booting stage was strong (grade 3), moderate (grade 5) and weak (grade 7) were 2, 15 and 3 in 20 substitution lines, Which accounted for 10%, 75% and 15% of the tested materials, respectively; (2) There was a significant positive correlation between cold tolerance at seedling stage and booting stage (p <0.05); 2 resistant grade 3 materials And DC866) not only can be used as seedling cold resistance source, but also can be used as booting cold resistance source; (3) soluble protein, soluble sugar, free amino acids, proline, malondialdehyde and superoxide dismutase There was no significant correlation between each physiological index and relative seed setting rate, so it was not suitable as the identification index of cold tolerance at booting stage.