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本文提出了一种同时检测粮食和饲料中七种主要单端孢霉烯族真菌毒素的电子捕获气相色谱分析方法.粮食样品经醋酸乙酯、氯仿、乙醇提取后,减压浓缩.浓缩液用活性炭-中性氧化铝(2:1,W/W)柱净化,84％乙腈水溶液洗脱.洗脱液减压浓缩至于,残渣用N-七氟正丁酰咪唑衍生化.电子捕获气相色谱的分析结果表明,活性炭-中性氧化铝柱能有效地除去粮食中的干扰物,有很好的分离效果.T-2毒素、HT-2毒素、二乙酰藨草镰刀菌烯醇、新茄病镰刀菌烯醇、脱氧雪腐镰刀菌烯醇、雪腐镰刀菌烯醇、镰刀菌烯酮-X等七种毒素在小麦、大麦、玉米及大米中的回收率在浓度范围为50ng/g—500ng/g的范围内均为80％以上.
In this paper, an electron capture gas chromatographic method for the simultaneous determination of seven major trichothecene mycotoxins in food and feedstuffs was proposed. Food samples were extracted with ethyl acetate, chloroform and ethanol and concentrated under reduced pressure. Purification by activated charcoal-neutral alumina (2: 1, W / W) column, eluted with 84% acetonitrile in water The eluate was concentrated under reduced pressure and the residue was derivatized with N- heptafluoro-n-butyrylimidazole Electron capture gas chromatography The analysis results show that activated carbon - neutral alumina column can effectively remove the interference of food, there is a good separation effect.T-2 toxins, HT-2 toxins, diacetyl tricinfluenza enol, The recoveries of seven toxins such as Fusarium enolase, deoxynivalenol, Fusarium oxysporum fusarium enkephalin-X and other toxins in wheat, barley, corn and rice ranged from 50ng / g It is 80% or more in the range of -500 ng / g.