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目的观察大脑中动脉缺血再灌注(MCAO/R)大鼠模型神经元中细丝蛋白(filamin,FLN)表达的变化情况,结合其超微结构改变,了解脑血管病变后神经元结构功能变化与FLN表达的相关性。方法制备MCAO/R大鼠模型,依照再灌注时间的不同分为2h、6h、12h、24h、3d及7d共6组,正常大鼠做对照组。按时相点取材,草酸蛳焦锑酸钾电镜观察,应用免疫组织化学方法染色并进行图像分析。结果除正常对照组外,其他各组神经元中均有阳性反应,12h、24h、3d组神经元中FLN表达较多,以3d时最明显。阳性神经元细胞集中在缺血半球皮质区。神经元中FLN阳性部位为胞浆的核周边部,表达均匀一致。胶质细胞中未见表达。结论再灌注损伤可引起神经元中细丝蛋白免疫阳性的表达,并与损伤时间的变化及神经元形态学改变有一定相关性,提示此现象可能与神经元的再生、修复及移行有关,有进一步研究的价值。
Objective To observe the changes of the expression of filamin (FLN) in the rat middle cerebral artery occlusion (MCAO / R) model and to investigate the ultrastructural changes of the neurons in cerebrovascular ischemia-reperfusion (MCAO / R) Correlation with FLN expression. Methods MCAO / R rat models were divided into 6h, 6h, 12h, 24h, 3d and 7d groups according to the different reperfusion time. Normal rats served as the control group. Draw on time, oxalic acid 蛳 potassium antimoniate potassium electron microscopy, immunohistochemistry staining and image analysis. Results In addition to the normal control group, neurons in other groups were positive, FLN expression in neurons at 12h, 24h, 3d group were more obvious, the most obvious at 3d. Positive neurons are concentrated in the ischemic hemisphere cortex. FLN positive neurons in the cytoplasm of the perinuclear area, the expression of uniform. No expression in glial cells. Conclusion Reperfusion injury can cause the expression of filament protein immunoreactive in neurons, and it has some correlation with the change of injury time and morphological changes of neurons, suggesting that this phenomenon may be related to the regeneration, repair and migration of neurons. There are The value of further research.