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4′6=脒基-2-苯基吲哚(DAPI)是一种荧光染料,和 DNA 形成复合物后发生强烈荧光。作者用感染伯氏疟原虫约氏变种的小鼠血,感染鹇疟原虫的鸭血及感染间日疟原虫的人血进行实验。血涂片用70%乙醇固定5分钟,空气中干燥后在1. 0微克/毫升 DAPI溶液(溶于双蒸水)中室温孵育15～30分钟,晾干后,用荧光显微镜检查。血涂片以固定于70%乙醇的效果最好.不固定、用更高浓度的乙醇或用甲醇固定都能降低荧光强度。
4’6 = Amidino-2-phenylindole (DAPI) is a fluorescent dye that fluoresces strongly when complexed with DNA. The authors performed experiments with mouse blood that infected Plasmodium berghei, the duck blood infected with Plasmodium falciparum, and human blood infected with P. vivax. Blood smear was fixed with 70% ethanol for 5 minutes, dried in air at 1.0 micrograms / ml DAPI solution (dissolved in double distilled water) for 15 to 30 minutes at room temperature, dried, and examined by fluorescence microscopy. Blood smear to the best effect fixed in 70% ethanol.No fixed, with a higher concentration of ethanol or fixed with methanol can reduce the fluorescence intensity.