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目的：探讨血管生成及细胞凋亡与喉粘膜上皮癌变的关系．方法：应用免疫组化和原位末端标记（ＩＳＥＬ）方法分别检测正常喉粘膜（Ｎ）、炎症（ＩＦ）、不典型增生（ＤＹＳ）和鳞癌（ＳＣＣ）中间质的微血管数量及上皮的细胞凋亡情况，标记后的血管和凋亡细胞通过计数分别以微血管密度（ＭＶＤ）及凋亡指数（ＡＩ）表示．结果：ＭＶＤ值从Ｎ，ＩＦ，ＤＹＳ至ＳＣＣ依次增高，ＤＹＳ及ＳＣＣ组的ＭＶＤ值与Ｎ，ＩＦ组存在显著性差异（Ｐ＜０．０５）；ＤＹＳ组的凋亡指数（ＡＩ）在所有病变中最高；ＤＹＳ及ＳＣＣ组的ＡＩ值较正常Ｎ及ＩＦ组明显增多，且有显著性差异（Ｐ＜０．０５）．结论：血管生成及细胞凋亡与喉粘膜癌前病变和癌变关系密切，在喉粘膜上皮癌变发生中起着重要的作用．
Objective: To investigate the relationship between angiogenesis and apoptosis and laryngeal mucosal carcinogenesis. Methods: Immunohistochemistry and ISEL were used to detect the number of microvessels and the number of epithelial cells in the interstitium of normal laryngeal mucosa (N), inflammation (IF), dysplasia (DYS) and squamous cell carcinoma (SCC) Apoptosis, labeled blood vessels and apoptotic cells were counted by microvessel density (MVD) and apoptotic index (AI), respectively. Results: The MVD values increased from N, IF, DYS to SCC in turn, and the MVD values in DYS and SCC groups were significantly different from those in N and IF groups (P <0.05). The apoptosis index (AI) The highest AI in DYS and SCC group was significantly higher than that in normal N and IF group (P <0.05). Conclusion: Angiogenesis and apoptosis are closely related to precancerous lesions and carcinogenesis of laryngeal mucosa, and play an important role in carcinogenesis of laryngeal mucosa.