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利用1个致病能力非常弱的稻瘟病菌菌株CY2和农杆菌介导T-DNA方法,已成功地获得了5000多个转化子,并以多个转化子混合接种含25个不同抗病基因的近等基因系,获得了30个致病突变体。但突变体针对不同的无毒基因有很大差别,从含有Pi-ta2的F128-1上获得了14个突变体,从含有Pi-a的IRBL-2上获得了7个突变体,从含有Pik-s的IRBL-4上获得了2个突变体,而从其它抗病基因的品系上未发现任何突变体,故T-DNA似乎有插入热点。不同突变体在携带相同抗病基因的品系上的致病性存在很大差异,表明这些近等基因系可能还携带其它抗稻瘟病基因。CY2不能侵染普遍认为不含抗稻瘟病基因的丽江新团黑谷(LTH),而7个突变体则能成功地使其发病,表明LTH亦含有抗稻瘟病基因。
More than 5000 transformants have been successfully obtained using one pathogenicity-less strain of Pyricularia grisea CY2 and Agrobacterium tumefaciens, and multiple transformants were inoculated with 25 different resistance genes Of nearly isogenic lines, access to 30 pathogenic mutants. However, the mutants showed great differences in different non-toxic genes. 14 mutants were obtained from F128-1 containing Pi-ta2, 7 mutants were obtained from IRBL-2 containing Pi-a, Two mutants were obtained on IRBL-4 of Pik-s and no mutants were found on the other disease-resistant lines, so T-DNA seems to have hot spots inserted. The pathogenicity of different mutants on strains carrying the same resistance genes is quite different, suggesting that these near isogenic lines may also carry other blast resistance genes. CY2 was not able to infect Lijiang Xintuanheigu (LTH), which is generally thought to contain no blast resistance gene, while seven mutants were able to successfully induce it, indicating that LTH also contains the blast resistance gene.