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目的 探讨碘在Graves病 (GD)发病中的作用。方法 取手术切除的GD患者甲状腺组织及甲状腺腺瘤患者腺瘤旁正常甲状腺组织 ,进行细胞培养 ,培养液中含不同浓度的NaⅠ (0～ 1mg/L)。采用免疫印迹技术 ,观察NaⅠ对甲状腺上皮细胞 (TEC)HLA DR表达的影响 ,并以扫描电镜观察NaⅠ处理后TEC的形态学改变。结果 (1)GDTEC :培养液中含NaⅠ浓度超过 10 0 μg/LHLA DR表达增加 (P <0 .0 1) ,其增加量与NaⅠ浓度无剂量依赖关系。扫描电镜显示培养液中NaⅠ浓度超过 10 0 μg/L于加碘 12h出现甲状腺细胞膜损伤。 (2 )腺瘤旁正常TEC :NaⅠ不同浓度组均无HLA DR表达。NaⅠ浓度超过 10 0 μg/L时 ,扫描电镜未显示TEC有细胞膜的损伤。 结论 过多的碘不仅造成GD甲状腺细胞膜的直接损伤 ,且可以上调TECHLA DR表达
Objective To investigate the role of iodine in the pathogenesis of Graves’ disease (GD). Methods The thyroid tissue of patients with GD and the normal thyroid tissue of adenoma of thyroid adenoma were collected and cultured. The culture medium contained different concentrations of NaⅠ (0 ~ 1 mg / L). Immunoblotting was used to observe the effect of NaⅠ on the expression of HLA DR in thyroid epithelial cells (TEC). The morphological changes of TEC after NaⅠ treatment were observed by scanning electron microscopy. Results (1) GDTEC: The concentration of NaI in culture medium exceeded 10 0 μg / LHLA DR increased (P <0.01), and the increase was not related to the concentration of NaⅠ. Scanning electron microscopy showed that the NaI concentration in the culture medium exceeded 100 μg / L, and thyroid cell membrane damage occurred 12 h after iodine addition. (2) Normal breast adenocarcinoma TEC: There was no HLA DR expression in different concentrations of NaⅠ. Scanning electron microscopy did not show TEC cell membrane damage when NaI concentrations exceeded 10 0 μg / L. Conclusions Excessive iodine not only directly damages GD thyroid cell membrane, but also up-regulates TECHLA DR expression