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目的探讨槲皮素对氧化应激大鼠肝细胞代谢的影响。方法用无血清培养液原代培养大鼠肝细胞,以H2O2诱导氧化应激。培养细胞分为空白对照组(仅用培养液培养)、槲皮素组(以20μmol/L槲皮素处理肝细胞24h)、H2O2组(以8.8mmol/LH2O2作用6h诱导细胞产生氧化应激)、槲皮素+H2O2组(先用20μmol/L槲皮素预处理24h后,再用8.8mmol/LH2O2诱导氧化应激)4组。实验结束后,收集各组培养液,离心,以核磁共振谱仪检测培养液中代谢物的变化情况。结果与空白对照组比较,最显著的变化包括:(1)槲皮素和槲皮素+H2O2组二甲胺含量增加;(2)H2O2、槲皮素和槲皮素+H2O2组乙酸含量增加,丙酮酸含量减少。其次的变化包括:(1)槲皮素组乳酸减少,谷胺酰胺增加;(2)H2O2组乳酸、牛磺酸增加,组氨酸减少;(3)槲皮素+H2O2组乳酸、牛磺酸和谷胺酰胺增加,组氨酸减少。结论槲皮素可通过加速糖酵解、促进脂肪酸氧化供能发挥抗氧化应激作用,对某些含氮化合物的代谢也具有调节作用。
Objective To investigate the effect of quercetin on hepatocyte metabolism in oxidative stress rats. Methods Primary cultured rat hepatocytes were cultured in serum-free medium and oxidative stress induced by H2O2. The cultured cells were divided into blank control group (culture medium only), quercetin group (treated with 20μmol / L quercetin for 24h), H2O2 group (induced by 8.8mmol / LH2O2 for 6h) , Quercetin + H2O2 group (pretreated with 20μmol / L quercetin for 24 hours and then induced oxidative stress with 8.8mmol / LH2O2). After the experiment, each group of culture media was collected and centrifuged, and the changes of metabolites in the culture solution were detected by nuclear magnetic resonance spectroscopy. Results Compared with the blank control group, the most significant changes included: (1) the increase of dimethylamine content in quercetin and quercetin + H2O2 groups; (2) the increase of acetic acid content in H2O2, quercetin and quercetin + H2O2 groups , Pyruvate content decreased. (2) Lactic acid and taurine increased and histidine decreased in H2O2 group; (3) Lactic acid and taurine in quercetin + H2O2 group increased Acid and glutamine increase, histidine decreased. Conclusion Quercetin can accelerate the glycolysis and promote the oxidation of fatty acids to exert anti-oxidative stress, and also regulate the metabolism of some nitrogen-containing compounds.