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本研究表明,抗CD_3McAb具有双向调控T淋巴细胞的功能,对多种疾病,包括恶性肿瘤有明显的临床疗效,作者用DNA重组技术将鼠源抗CD_3McAb在基因水平改建为鼠/人嵌合抗体使之更适合临床应用时,用PCR法从HIT_(3a)(抗CD_3McAb)杂交瘤细胞株中分离出Ig重、轻链可变区基因,並分别克隆进了pGEM-7Zf(+),pGEM-3Zf(+)载体中,重组质粒DNA序列测定
This study showed that anti-CD_3McAb has the function of bidirectional regulation of T lymphocytes and has obvious clinical curative effect on various diseases, including malignant tumors. The recombinant DNA technology of mouse anti-CD_3McAb was used to reconstruct murine anti-CD_3McAb into mouse / human chimeric antibody To make it more suitable for clinical applications, Ig heavy and light chain variable region genes were isolated from HIT_ (3a) (anti-CD_3McAb) hybridoma cell lines by PCR and cloned into pGEM-7Zf (+), pGEM -3Zf (+) vector, sequencing of recombinant plasmid DNA