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本实验用磺化四苯基卟啉作柱前衍生试剂,研究了在Hg(Ⅱ)催化下,于pH5.5时,TPPS_4与Mn(Ⅱ)、Zn(Ⅱ)、Cu(Ⅱ)的配位反应,提出了以苄基三乙基氯化铵作离子对试剂,反相离子对高效液相色谱-光度法分离和测定痕量Mn(Ⅱ)、Zn(Ⅱ)、Cu(Ⅱ)的新方法,检测下限(×10~(-9)g/mL):Mn~(2+)0.15,Zn~(2+)0.20,Cu~(2+)0.11。所确立的方法用于茶汤中锰、锌、铜的测定,结果满意。
In this experiment, we used sulfonated tetraphenylporphyrin as pre-column derivatization reagent to study the effect of TPPS_4 and Mn (Ⅱ), Zn (Ⅱ) and Cu (Ⅱ) at pH5.5 catalyzed by Hg (Ⅱ) Reaction was proposed to separate and determine trace Mn (Ⅱ), Zn (Ⅱ) and Cu (Ⅱ) with benzyltriethylammonium chloride as ion pair reagent and reversed phase ion pair high performance liquid chromatography coupled with spectrophotometry The new method, the detection limit (× 10 ~ (-9) g / mL): Mn 2+, Zn 2+ 0.20, Cu 2+ 0.11. The established method was applied to the determination of manganese, zinc and copper in tea soup with satisfactory results.