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本文旨在证明一种乙基睾丸酮的类似物-丹那唑在体内和体外对黄体功能的抑制作用。作者选用体重250～300g的雌性大鼠,与成年雄鼠合笼,以阴道细胞涂片中查到精子之日算作妊娠的零日。在体实验于妊娠第十至第十五日,每8小时或24小时注射1次丹那唑的芝麻油混悬液,并在妊娠第十至第十八日,每48小时取血1次,用放射免疫方法测定血清孕酮水平。离体实验用病人黄体及妊娠第十天的大鼠黄体,用胶原酶处理成细胞制备,在含牛血清的基本孵育液中孵育2日,孵育液中分别加入不同剂量的丹那唑,每日更换孵育液,并测定其中孕酮含量。
The purpose of this article is to demonstrate the inhibitory effect of danazol, an analog of ethyltestosterone, on luteal function in vivo and in vitro. The author chooses the weight of 250 ~ 300g female rats, and adult male rats caged cage, vaginal smear found sperm day counted as a day of pregnancy. In vivo experiments on the tenth to the fifteenth day of pregnancy, once every 8 hours or 24 hours injection of danazol sesame oil suspension, and in pregnancy from the 10th to the 18th, once every 48 hours to take blood with Radioimmunoassay for serum progesterone levels. In vitro experiments with the corpus luteum and the tenth day of pregnancy, corpus luteum, collagenase treatment into cells prepared in the basic incubation with bovine serum incubated for 2 days, respectively, incubated with different doses of Danazol, added daily The incubation solution was changed and the progesterone content was determined.