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目的探讨细胞周期检测点激酶1(Chk1)在胃腺癌组织中的表达及其意义。方法采用免疫组化、原位杂交方法检测80例胃腺癌组织和80例正常胃黏膜组织中Chk1蛋白及mRNA的表达,并分析Chk1表达与胃癌临床病理参数的关系。结果胃腺癌组织和正常胃黏膜组织中Chk1蛋白阳性表达率分别为78.75%(63/80)和45.00%(36/80),两组相比,差异有显著性(P<0.05)。Chk1mRNA在胃腺癌组织和正常胃黏膜组织中的阳性表达率分别为82.50%(66/80)和40.00%(32/80),两组差异具有统计学意义(P<0.05),Chk1蛋白及mRNA表达与胃癌浸润和淋巴结转移有关(P<0.05)。结论 Chk1表达与胃腺癌发生、发展密切相关。
Objective To investigate the expression and significance of the cell cycle checkpoint kinase 1 (Chk1) in gastric adenocarcinoma. Methods The expressions of Chk1 protein and mRNA in 80 cases of gastric adenocarcinoma and 80 cases of normal gastric mucosa were detected by immunohistochemistry and in situ hybridization. The relationship between Chk1 expression and clinicopathological parameters was analyzed. Results The positive rates of Chk1 protein in gastric adenocarcinoma and normal gastric mucosa were 78.75% (63/80) and 45.00% (36/80), respectively. There was significant difference between the two groups (P <0.05). The positive expression rates of Chk1mRNA in gastric adenocarcinoma and normal gastric mucosa were 82.50% (66/80) and 40.00% (32/80), respectively, with significant difference between the two groups (P <0.05). Chk1 protein and mRNA The expression was related to gastric cancer infiltration and lymph node metastasis (P <0.05). Conclusion The expression of Chk1 is closely related to the occurrence and development of gastric adenocarcinoma.