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为探讨外源多胺对花生(Arachis hypogaea)抗盐性的影响,以盆栽花生‘花育22’为试验材料,通过叶面喷施1mmol·L~(–1)腐胺(Put)、1 mmol·L~(–1)亚精胺(Spd)、1 mmol·L~(–1)精胺(Spm)的方法,研究多胺对150 mmol·L~(–1) NaCl胁迫下盆栽花生的缓解作用。结果表明,与对照(CK)相比,盐胁迫显著抑制了花生植株的生长与荚果产量,降低了叶绿素含量和抗氧化酶活性,丙二醛(MDA)含量、叶片相对电导率增加;在盐胁迫下,叶面喷施Put、Spd、Spm处理均可有效促进花生植株的生长,提高了超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性,增强植株抗氧化能力,显著降低了花生叶片相对电导率和MDA的积累量,从而缓解盐胁迫对质膜的过氧化伤害;提高了叶绿素含量,促进了植株高度的生长与分支数增多,增加了干物质积累量,从而提高了花生荚果产量;其中,Spm处理引起的变化幅度大于Spd和Put处理。研究结果说明,多胺有利于花生幼苗在盐胁迫下活性氧代谢和光合色素含量的提高,促进花生植株的生长,降低盐胁迫对花生植株的抑制作用,且Spm处理的效果最好。
In order to investigate the effects of exogenous polyamines on the salt resistance of Arachis hypogaea, potted peanut ’Huayu 22’ was used as test material and 1 mmol·L -1 putrescine (Put), 1 (-1) Spd and 1 mmol·L -1 Spm to study the effect of polyamine on the growth of potted peanut under 150 mmol·L -1 NaCl stress. Ease the role. The results showed that compared with CK, salt stress significantly inhibited the growth and pod yield of peanut, reduced the content of chlorophyll and antioxidant enzyme activity, the content of malondialdehyde (MDA) and the relative conductivity of leaves, Under stress, Put, Spd and Spm could promote the growth of peanut and increase the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) , Enhanced the antioxidant capacity of plants, significantly reduced the relative electrical conductivity and the accumulation of MDA in the peanut leaves, thereby alleviating the damage caused by salt stress on the plasma membrane and increasing the content of chlorophyll, increasing the plant height and branch number, and increasing Dry matter accumulation, thereby increasing the peanut pod yield; Among them, Spm treatment caused more than the magnitude of change Spd and Put treatment. The results showed that polyamines were beneficial to the increase of reactive oxygen species metabolism and photosynthetic pigment content of peanut seedlings under salt stress, promoted the growth of peanut plants, reduced the inhibitory effect of salt stress on peanut plants, and the effect of Spm was the best.