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采用单克隆抗体包裹超顺磁性聚合体微球技术分离标有抗体的精子。在磁性微球处理前后作精子的免疫珠结合,评价带有自身抗体精子的百分率以及该技术对精液参数的影响,旨在探讨分离与抗体结合精于后的无抗体精子悬液用于体外或体内授精的可行性。用直接与间接免疫珠结合试验选择11例精子自身免疫患者,4例取精液、7例采血清。当精液和血清与无抗体精子(取自7名健康供精者)孵育,免疫珠结合率为30％～80%。标记抗体精子免疫珠结合试验操作按Busacca等所描述。自身免疫精子标本或已吸附血清抗精子抗体的无抗体精子在
Antibody-coated sperm were isolated using monoclonal antibody-coated superparamagnetic polymer microspheres. Before and after the treatment of magnetic microspheres as sperm immunoglobulin binding, the evaluation of the percentage of sperm with autoantibodies and the impact of the technology on semen parameters, to explore the isolation and antibody binding of antibody-free sperm suspension for in vitro or Feasibility of in vivo insemination. Eleven patients with autoimmune spermatozoa were selected by direct and indirect immunobead binding assay. Four patients received semen and seven patients received serum. When semen and serum were incubated with antibody-free sperm (from seven healthy donors), the immunobead binding rate was 30% to 80%. Labeled Antibodies Sperm Immunoglobulin Binding Assay Procedures are as described by Busacca et al. Autoimmune sperm samples or antibodies that have adsorbed serum anti-sperm antibody-free sperm