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目的:建立伯氏疟原虫青蒿素抗性系鼠疟模型。方法:采用鼠-鼠间血传、青蒿素剂量递增法对伯氏疟原虫常规株(青蒿素敏感株)进行抗性培育,通过测定伯氏疟原虫青蒿素抗性株和常规株的ED50,计算抗性指数。结果:从青蒿素起始剂量126.2 mg/kg(相当于1/2 ED50)开始,目前已培育至60代,青蒿素用药剂量达2646mg/kg。第16、24、34、42代及第53代抗性指数(RI)逐渐上升,分别为3.33、8.31、12.41、14.15、15.68。结论:建立了具有中度抗性的伯氏疟原虫青蒿素抗性系鼠疟模型。
Objective: To establish a mouse model of Plasmodium berghei resistant artemisinin resistance in mice. Methods: The common strains of artemisinin-resistant bermudagrass (Artemisinin-sensitive strains) were cultivated by means of rat-mouse intercourse and artemisinin dose escalation. The resistance of Plasmodium berghei artemisinin- ED50, calculate the resistance index. Results: Starting from the initial dose of artemisinin 126.2 mg / kg (equivalent to 1/2 ED50), it has been cultivated to 60 generations and the dose of artemisinin is 2646 mg / kg. The resistance index (RI) at the 16th, 24th, 34th, 42nd generation and 53rd generation increased gradually and reached 3.33, 8.31, 12.41, 14.15 and 15.68 respectively. Conclusion: The model of Plasmodium berghei resistant artemisinin resistant mouse malaria was established with moderate resistance.