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目的:探讨JNK/SAPK和NF-κB/IκB信号通路在重症急性胰腺炎(SAP)肺损伤中的作用及ICAM-1的表达和意义。方法:将60只SD大鼠随机分为对照组和SAP组,每组30只,采用5%牛磺胆酸钠逆行胰胆管注射建立SAP模型。于造模后3h、6h、12h观察2组肺组织病理变化并进行肺损伤评分,检测肺组织湿干重比(W/D),ELISA方法检测TNF-α、IL-1β表达水平,RT-PCR方法检测ICAM-1的表达量,蛋白印迹(Western Blot)法检测肺组织JNK蛋白磷酸化(p-JNK)、IκBα磷酸化(p-IκBα)蛋白的表达含量。结果:SAP组的病理评分、肺W/D值、TNF-α、IL-1β表达量、ICAM-1mRNA、p-JNK和p-IκBα表达量均显著高于对照组(P<0.05);随着损伤时间的延长,各指标有升高的趋势。通过多元相关性分析发现ICAM-1mRNA、p-JNK、p-IκBα的表达水平与TNF-α、IL-1β的表达水平呈明显正相关(P<0.05)。结论:SAP大鼠肺组织ICAM-1 mRNA、p-JNK和pIκBα表达水平随着肺损伤程度的增加而增高,ICAM-1分子的过度表达,JNK/SAPK和NF-κB/IκB信号通路的激活是SAP肺损伤的重要发病机制。
Objective: To investigate the role of JNK / SAPK and NF-κB / IκB signaling pathway in lung injury of severe acute pancreatitis (SAP) and the significance of ICAM-1 expression. Methods: Sixty SD rats were randomly divided into control group and SAP group, 30 rats in each group. SAP model was established by injecting 5% sodium taurocholate into pancreaticobiliary duct. The pathological changes of the lungs were observed at 3h, 6h, 12h after operation and the lung injury scores were scored. The wet / dry weight ratio (W / D) of lung tissue was measured. The expression of TNF-α and IL- The expression of ICAM-1 was detected by PCR. The expressions of p-JNK and p-IκBα in lung tissue were detected by Western Blot. Results: The pathological score, W / D of lung, expression of TNF-α, IL-1β, ICAM-1mRNA, p-JNK and p-IκBα in SAP group were significantly higher than those in control group With the extension of the injury time, each indicator has an increasing trend. The multivariate correlation analysis showed that the expression levels of ICAM-1mRNA, p-JNK and p-IκBα were positively correlated with the expression of TNF-α and IL-1β (P <0.05). CONCLUSIONS: The expression of ICAM-1 mRNA, p-JNK and pIκBα in lung tissue of SAP rats increased with the increase of lung injury, over-expression of ICAM-1, activation of JNK / SAPK and NF-κB / IκB signaling pathway Is an important pathogenesis of SAP lung injury.