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目的 克隆全反式维甲酸诱导前列腺癌细胞系 DU- 1 45细胞产生凋亡的相关基因。探讨维甲酸诱导前列腺癌细胞产生凋亡的分子机理。方法 应用全反式维甲酸诱导前列腺癌细胞系DU- 1 45细胞凋亡 ,采用基于 PCR的改良消减杂交技术克隆与凋亡相关的基因。结果 在前列腺癌 DU- 1 45细胞凋亡过程中 ,有大量肿瘤坏死因子 (TNF)的表达 ,同时 ,克隆出 c- erb B- 2基因。结论 TNF及 c- erb B- 2基因在由全反式维甲酸诱导的前列腺癌细胞系 DU- 1 45细胞凋亡过程中起着重要作用
Objective To clone the gene related to apoptosis induced by all-trans retinoic acid in prostate cancer cell line DU-1 45. To investigate the molecular mechanism of retinoic acid-induced apoptosis in prostate cancer cells. Methods Apoptosis of prostate cancer cell line DU-1 45 cells was induced by all-trans retinoic acid. Apoptosis-related genes were cloned by PCR-based modified subtractive hybridization. Results In the process of apoptosis of prostate cancer DU-1 45 cells, a large amount of tumor necrosis factor (TNF) was expressed. At the same time, the c-erb B-2 gene was cloned. Conclusion The TNF and c-erb B-2 genes play an important role in the apoptosis of DU-1 45 cells induced by all-trans retinoic acid