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目的:建立高效液相色谱法测定救尔心胶囊中丹参素钠含量的方法。方法:色谱柱为Phenomenex C_(18)柱(250 mm×4.6 mm,5μm),以甲醇-1%醋酸溶液(5:95)为流动相,检测波长为280 nm,流速为0.8 ml·min~(-1),柱温为22℃。结果:丹参素钠在8.0~200.2μg·ml~(-1)范围内线性关系良好(r=0.999 9),平均加样回收率为99.4%(RSD=0.7%)。结论:该方法简便、准确,重复性好,可作为救尔心胶囊中丹参素钠的含量测定方法。
Objective: To establish a method for the determination of Danshensu sodium in Xi’er heart capsule by high performance liquid chromatography. METHODS: The mobile phase was Phenomenex C_ (18) column (250 mm × 4.6 mm, 5 μm) with methanol-1% acetic acid solution (5:95) as the mobile phase. The detection wavelength was 280 nm and the flow rate was 0.8 ml · min ~ (-1) at a column temperature of 22 ° C. Results: The sodium danshensu showed good linearity (r = 0.999 9) in the range of 8.0 ~ 200.2 μg · ml ~ (-1) with an average recovery of 99.4% (RSD = 0.7%). Conclusion: The method is simple, accurate and reproducible, and can be used as a method for the determination of Danshensu sodium in Xier Er Xin Capsules.