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将小叶杨×美杨、小叶杨×胡杨、小叶杨×大叶杨、小叶杨×椅杨、箭杆杨×大叶杨和741×[银白杨×(山杨×小叶杨)]的杂种胚珠进行离体培养,全部成功地获得了杂种植株。以1/2MS为基本培养基,进行了下列试验:1.不同浓度IAA和BA对诱导不定芽和绿苗发生的效应,结果最适分化培养基为1/2MS+IAA0.01mg/1+BA0.1mg/1;除去IAA,仅附加BA0.1mg/1,也是有效的;2.不同浓度NAA和IBA对生根的影响,试验证明最适生根培养基为1/2MS+NAA0.01—0.02mg/1或1/2MS+IBA0.1—0.5mg/1。接种时杂种胚珠内胚的发育阶段对萌发率及以后的生长有显著影响:含早期心形胚的杂种胚珠,萌发率只有33％,小苗弱而且很难长大;含中期或后期心形胚、鱼雷胚及子叶伸长期幼胚的杂种胚珠,萌发率在50％以上,最高可达100％,小苗健壮,容易分化芽和绿苗丛。胚根端和下胚轴的横切面显示:表皮、皮层、内皮层和中柱鞘的细胞脱分化成为分生细胞。这些分生细胞分裂活动的结果,形成了不定芽。
Populus × Populus simon × Populus × Populus euphratica × Populus × Populus × Populus × Populus × Populus × Populus × Populus × Populus × Populus × Populus euphratica × 741 × Poplar × Populus × Populus × Populus trichocarpa) hybrid ovules In vitro culture, all successfully obtained hybrid plants. 1 / 2MS as the basic medium, the following tests were carried out: 1. Effect of different concentrations of IAA and BA on the induction of adventitious shoots and green shoots, the results of the best medium for the differentiation of 1 / 2MS + IAA0.01mg / 1 + BA0 .1mg / 1; IAA is only added, BA0.1mg / 1 is also effective; 2.The effect of different concentrations of NAA and IBA on rooting, the test proved that the optimum rooting medium is 1 / 2MS + NAA0.01-0.02mg / 1 or 1 / 2MS + IBA 0.1-0.5 mg / 1. During the inoculation, the stage of embryo development in hybrid ovules had a significant effect on the germination rate and subsequent growth: the hybrid ovule with early heart-shaped embryos had a germination rate of only 33%, the seedlings were weak and difficult to grow; the mid- , Torpedo embryo and cotyledon embryo hybrid embryo elongation embryos, germination rate of 50% or more, up to 100%, seedlings robust, easy to differentiate shoots and green seedlings. Cross-sections of the radicle and hypocotyls show that cells of the epidermis, cortex, endothelium, and pericycle are dedifferentiated into meristematic cells. As a result of these meristematic cell divisions, adventitious buds are formed.