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导数光谱法是解决可见——紫外光谱干扰的新方法。该法原理简单、操作方便。本文分别在附有导数装置的 UV-300型自记式分光光度计及普通的手动式751G 型分光光度计上用一阶导数法测定了常药降压片中氢氯噻嗪的含量。其回收率分别为99.51±0.47%及99.14±0.45%(置信率95%)。
Derivative spectroscopy is a new method to solve the visible-ultraviolet spectrum interference. The principle is simple, easy to operate. In this paper, the first derivative method was used to determine the content of hydrochlorothiazide in the conventional antihypertensive tablets by UV-300 self-recording spectrophotometer with a derivative device and the ordinary manual 751G spectrophotometer. The recoveries were 99.51 ± 0.47% and 99.14 ± 0.45%, respectively (95% confidence interval).