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目的:研究菝葜Smilax china L.的甾体皂苷类成分及其细胞毒活性。方法:利用色谱技术进行分离纯化,并根据化合物的理化性质和光谱数据进行结构鉴定。采用MTT法对分离得到的单体化合物进行细胞毒活性的初筛。结果:从该植物乙醇提取物的正丁醇部分分离得到6个甾体皂苷,分别鉴定为borassoside B(1),薯蓣皂苷(2),原薯蓣皂苷(3),22-O-甲基原薯蓣皂苷(4),isonarthogenin 3-O-α-L-rhamnopyranosyl(1-2)-O-[α-L-rhamnopyranosyl-(1-4)]-β-D-glucopyranoside(5),diosgenin-3-O-[α-L-rha(1-3)-α-L-rha(1-4)-α-L-rha(1-4)]-β-D-glucopyranoside(6)。在样品溶液质量浓度为5 mg.L-1时,化合物2和6对K-562癌细胞生长的抑制率分别是89.1%9,0.1%。结论:化合物1和6是首次从该植物中分离得到,也是首次从菝葜属植物中分离得到。化合物2和6对K-562癌细胞具有明显的体外细胞毒活性。
Objective: To study the steroidal saponins of Smilax china L. and its cytotoxic activity. Methods: The compounds were separated and purified by chromatographic techniques and their structures were elucidated on the basis of physicochemical properties and spectral data of the compounds. The cytotoxic activity of the isolated monomeric compounds was tested by MTT assay. Results: Six steroidal saponins were isolated from the n-butanol fraction of the plant ethanol extract and identified as borassoside B (1), diosgenin (2), protodioscin (3), 22-O-methyl Dioscin (4), isonarthogenin 3-O-α-L-rhamnopyranosyl (1-2) -O- [α-L-rhamnopyranosyl- (1-4)] - β-D-glucopyranoside -O- [α-L-rha (1-3) -α-L-rha (1-4) -α-L-rha (1-4)] - β-D-glucopyranoside (6). The inhibitory rates of compounds 2 and 6 on the growth of K-562 cancer cells were 89.1% 9 and 0.1% at the concentration of 5 mg.L-1 respectively. Conclusion: Compounds 1 and 6 were isolated from this plant for the first time and were also isolated for the first time from the Genus. Compounds 2 and 6 have significant cytotoxic activity against K-562 cancer cells in vitro.