目的：研究ＡＴＰ和Ｓ１４０对Ⅰ型和Ⅱ型核糖体失活蛋白（ＲＩＰ）失活核糖体的影响．方法：采用凝胶电泳分析特征ＲＩＰ作用片断（Ｒ片断），以及改进的二步法标记苯丙氨酸外源ｐｏｌｙ（Ｕ）翻译体系，定量检测外加因子对ＲＩＰ失活核糖体作用的影响．结果：ＡＴＰ和Ｓ１４０对所有用于检测的Ⅰ型和Ⅱ型ＲＩＰ失活核糖体的功能均表现出不同程度的促进作用．对于Ⅰ型ＲＩＰ中克木毒蛋白、天花粉毒蛋白、γ苦瓜子毒蛋白、丝瓜毒蛋白Ａ、丝瓜毒蛋白Ｓ和Ⅱ型ＲＩＰ中蓖麻毒蛋白、蓖麻毒蛋白Ａ链；辛纳毒蛋白、辛纳毒蛋白Ａ链，ＩＣ５０增强活性比率分别为３１０８，１５，１５１，４５，５１和４７，７，２６，１２．结论：首次发现ＡＴＰ和Ｓ１４０对Ⅱ型ＲＩＰ及其Ａ链具有活性增强作用．克木毒蛋白和辛纳毒蛋白在对ＡＴＰ和Ｓ１４０的辅助需求方面有显著区别 Objective: To investigate the effect of ATP and S140 on inactivation of ribosomes by type Ⅰ and type Ⅱ ribosome inactivating proteins (RIPs). Methods: The characteristic RIP fragment (Rsegment) was analyzed by gel electrophoresis, and the modified twophase method labeled phenylalanine exogenous poly (U) translation system was used to quantitatively detect the effect of extra factors on RIP inactivation of ribosome . Results: Both ATP and S-140 promoted the function of all types of RIP-inactivating ribosomes tested for type I and type II to varying degrees. In the type ¢ ñ RIP ricketoxin protein, trichosanthin protein, γ  bitter melon seed protein, loofah toxin A, loofah toxin S and Ⅱ type RIP ricin protein, ricin A chain; Toxic protein, SinA protein A chain, IC50 enhanced activity rates were 3108,15,151,45,51 and 47,7,26,12. Conclusion: It was first found that ATP and S140 have activity enhancing effect on type Ⅱ RIP and its Achain. Krypton and sinanein have significant differences in the auxiliary requirements for ATP and S-140