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采用薄层扫描法测定小蔓长春花组织培养物中长春胺的含量。应用高效硅胶Ｇ薄层板，展开剂为石油醚－氯仿－甲醇（１０：１０：１.２）。在λｓ＝４９０ｎｍ，λＲ＝５５０ｎｍ波长处扫描。长春胺标准品的线性范围为０.５３５～３.７４５μｇ；平均回收率为９８.６８％，ＲＳＤ＝１.９％（ｎ＝６）。小蔓长春花组织培养物经碱性氯仿和２％硫酸提取后点样，按随行外标两点法进行测定，计算样品的含量。测得在固体和液体培养基Ｂ５ａ上生长的由根、茎、叶诱导产生的培养物中长春胺含量分别为：０.６２±０.２２‰、０.３１±０.０７‰、０.３９±０.１０‰和０.５９±０.０９‰、０.５６±０.１５‰、０.５２±０.０７‰。
The content of vincamine in the tissue culture of Vinca minor was measured by TLC scanning. A high-efficiency silica gel G thin plate was used and the developing solvent was petroleum ether-chloroform-methanol (10:10:1.2). Scanned at a wavelength of λs=490 nm, λR=550 nm. The linear range of the vincamine standard was 0.535 to 3.745 μg; the average recovery was 98.68%, and the RSD was 1.9% (n=6). The tissue culture of Vinca minors was spotted after extraction with basic chloroform and 2% sulfuric acid. The content was determined according to the two-point method accompanied by external standards. The contents of vincamine in cultures induced from roots, stems, and leaves grown on solid and liquid media B5a were found to be 0.62±0.22 ‰, 0.31±0.07 ‰, 0, respectively. 39±0.10 ‰ and 0.59±0.09 ‰, 0.56±0.15 ‰, and 0.52±0.07 ‰.