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以猴艾滋病病毒ＳＩＶ作为人艾滋病病毒ＨＩＶ体外模型，观察了兴安升麻皂甙（Ｃｄ－Ｓ）在Ｈｕｔ－７８－ＳＩＶ体外培养系统对ＳＩＶ的抑制作用，并与阳性药叠氮胸苷（ＡＺＴ）的作用进行了比较。结果２００ｍｇ／ｍｌＣｄ－Ｓ可抑制荧光阳性细胞数。抑制率为２４．００％，ＳＩＶ产量下降２～３个单位，细胞病变程度亦有所缓解。与阳性药ＡＺＴ相比，Ｃｄ－Ｓ对ＳＩＶ的抑制作用仅为轻度。为探讨其作用机制，同时观察了Ｃｄ－Ｓ在体外ＰＨＡ刺激的淋巴细胞培养系统对３Ｈ－ＴｄＲ转运的影响，结果表明１７５．０μｇ／ｍｌ剂量Ｃｄ－Ｓ可明显抑制３Ｈ－ＴｄＲ的转运，抑制率达９３．８５％，提示Ｃｄ－Ｓ是通过抑制细胞膜的核苷转运过程，导致ＳＩＶ在宿主细胞内自身ＤＮＡ合成受限，ＳＩＶ产量下降，表现为Ｃｄ－Ｓ对ＳＩＶ的抑制作用。
Using monkey AIDS virus SIV as human HIV HIV in vitro model, we observed the inhibition of SIV by Huh-78-SIV in vitro culture system and the positive drug zidovudine (AZT). The role was compared. Results 200 mg/ml Cd-S inhibited the number of positive cells. The inhibition rate was 24.00%, SIV production decreased by 2 to 3 units, and the extent of cytopathy also eased. Compared with the positive drug AZT, the inhibitory effect of Cd-S on SIV was only mild. To investigate its mechanism of action, the effect of Cd-S on the 3H-TdR transport in PHA-stimulated lymphocyte culture system was also observed. The results showed that the dose of 175.0 μg/ml Cd-S could significantly inhibit the 3H-TdR transport and inhibit The rate reached 93.85%, suggesting that Cd-S inhibits the nucleoside transport process of the cell membrane, resulting in the limited synthesis of SIV in the host cell’s own DNA, and a decrease in the production of SIV, manifested as the inhibitory effect of Cd-S on SIV.