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目的:探讨地黄寡糖及其主要组分对H2O2所致大鼠大脑皮质和海马脑片氧化应激损伤的保护效果。方法:取SD大鼠大脑皮质和海马制作脑片,然后随机分为对照组(Control);单纯地黄寡糖用药组(ROS);单纯水苏糖组(stachyose);单纯梓醇组(catalpol);H2O2损伤组(H2O2);损伤前地黄寡糖预处理组(ROS+H2O2);损伤前水苏糖预处理组(stachyose+H2O2);损伤前梓醇预处理组(catalpol+H2O2)。TTC染色法检测脑片组织损伤百分率,比色法检测培养液中乳酸脱氢酶(LDH)含量。结果:H2O2孵育30min明显损伤大鼠海马和皮质脑片,组织损伤率明显升高,LDH释放增加(P<0.01)。8、40、200 mg/L的地黄寡糖或120 mg/L的水苏糖、20 mg/L的梓醇可不同程度改善H2O2所致的氧化应激损伤引起的组织损伤,减少LDH的漏出,但各成分均对对照组脑片无影响。结论:地黄寡糖、水苏糖、梓醇均能减轻H2O2所造成的氧化应激损伤。梓醇相比水苏糖在地黄寡糖中起主要作用。
Objective: To investigate the protective effect of Rehmannia glutinosa oligosaccharides and its major constituents on H2O2-induced oxidative stress in the cerebral cortex and hippocampus of rats. Methods: Brain slices were made from the cerebral cortex and hippocampus of SD rats and then randomly divided into control group, ROS group, stachyose group, catalpol group, Pretreatment group (H2O2); Pretreatment group Rehmannia glutinosa oligosaccharide (ROS + H2O2); Pre-injury stachyose + H2O2; Pretreatment catalpol + H2O2 group. TTC staining method was used to detect the percentage of brain tissue injury, and the content of lactate dehydrogenase (LDH) in culture medium was detected by colorimetric method. Results: After incubated with H2O2 for 30 minutes, the hippocampal and cortical slices of rats were significantly damaged. The damage rate of the tissue was significantly increased and the release of LDH was increased (P <0.01). 8, 40, 200 mg / L Rehmannia glutinosa or 120 mg / L stachyose and 20 mg / L catalpol can improve the tissue damage caused by H2O2-induced oxidative stress injury to a certain extent and reduce the leakage of LDH , But each component had no effect on the control group. Conclusion: Rehmannia glutinosa oligosaccharides, stachyose, catalpol can reduce oxidative stress induced by H2O2. Catalpol plays a major role in rehmannia oligosaccharides compared to stachyose.