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目的:探讨幽门螺杆菌对甲硝唑的耐药机制。方法:体外诱导甲硝唑耐药株,PCR扩增rdxA基因使其突变,应用双向凝胶电泳分离敏感株和耐药株蛋白质,寻找其差异性蛋白质。结果:利用倍比稀释试验构建幽门螺杆菌对甲硝唑的耐药株,其最低抑菌浓度为敏感株64倍。扫描蛋白表达图谱,共得到差异性蛋白质斑点55个。结论:运用双向电泳技术获得幽门螺杆菌敏感株与甲硝唑耐药株的差异蛋白,从蛋白质水平为研究幽门螺杆菌对甲硝唑耐药提供依据。
Objective: To investigate the mechanism of resistance of Helicobacter pylori to metronidazole. Methods: Metronidazole resistant strains were induced in vitro and the rdxA gene was amplified by PCR. The two proteins were separated by two - dimensional gel electrophoresis. Results: To construct the resistant strains of H. pylori to metronidazole by multiplicity dilution test, the minimum inhibitory concentration was 64 times of the sensitive strain. Scan protein expression profiles, a total of 55 spots of differential protein spots were obtained. Conclusion: The differential proteins of Helicobacter pylori-sensitive strains and metronidazole-resistant strains were obtained by two-dimensional gel electrophoresis. The protein level was used as the basis for the study of H. pylori resistance to metronidazole.