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目的:探讨血管紧张素转换酶基因(ACE)多态性与其血清水平及2型糖尿病(T2D)发生的相关性。方法:应用聚合酶链反应检测T2D患者287例和正常对照组307例健康人的ACE基因Alu重复序列的插入/缺失(I/D)多态性,采用全自动生化分析仪检测ACE活性及血脂水平,采用SPSS11.0软件包统计分析基因型分布和等位基因频率与其活性、血脂水平及T2D的相关性。结果:ACEI/D多态性在T2D组(DD:13.36%、ID:45.93%、II:40.72%)与对照组(DD:13.24%、ID:43.90%、II:42.86%)的基因频率无显著性差异(P>0.05)。T2D组ACE各基因型之间ACE活性有显著性差异(P<0.01)。T2D各基因型的血脂水平分析显示II型与DD型之间HDL有显著性差异(P<0.05)。结论:ACE基因DD型和D等位基因与ACE活性显著相关,但ACEI/D多态性不是T2DM发生的危险因素且无关,DD型与高HDL水平相关。
Objective: To investigate the relationship between angiotensin converting enzyme gene (ACE) polymorphisms and serum levels and type 2 diabetes mellitus (T2D). Methods: Polymerase chain reaction (PCR) was used to detect the Alu repeat / insertions (A / D) polymorphism of ACE gene in 287 T2D patients and 307 normal controls. ACE activity and serum lipids Level, using SPSS11.0 software package statistical analysis of genotype distribution and allele frequency and its activity, blood lipid levels and T2D correlation. Results: The frequency of ACEI / D polymorphism in T2D group (DD: 13.36%, ID: 45.93%, II: 40.72%) and control group (DD: 13.24%, ID: 43.90%, II: 42.86% Significant difference (P> 0.05). There was a significant difference in ACE activity between ACE genotypes in T2D group (P <0.01). Analysis of blood lipid levels in T2D genotypes showed a significant difference in HDL between type II and DD (P <0.05). CONCLUSIONS: The DD genotype and D allele of ACE gene are significantly correlated with ACE activity. However, ACEI / D polymorphism is not a risk factor for T2DM and is unrelated to DD genotype.