论文部分内容阅读
目的建立水产品中孔雀石绿和隐色孔雀石绿药物残留量同时测定的高效液相色谱-串联质谱方法。方法样品选择乙腈提取,用正己烷脱脂,中性氧化铝固相萃取柱净化,采用LC-MS/MS选择反应监测(SRM)正离子模式测定进行定性、定量分析。结果孔雀石绿和隐色孔雀石绿药物的检出限(LOD)为0.2μg/kg,定量限(LOQ)为0.4μg/kg,检测结果的相对标准偏差为1.31%~8.95%,加标回收率达到74.5%~108.3%。结论该方法具有比较高的重现性和选择性,在水产品中孔雀石绿和隐色孔雀石绿的残留测定中具有很好的应用前景。
OBJECTIVE To establish simultaneous determination of malachite green and leucomalachite green drug residues in aquatic products by high performance liquid chromatography-tandem mass spectrometry. Methods The samples were extracted with acetonitrile, cleaned up with n-hexane degreasing and neutral alumina solid-phase extraction (SPE) column, and qualitative and quantitative analysis was carried out by LC-MS / MS selective reaction monitoring (SRM) Results The detection limit (LOD) of malachite green and leucomalachite green drug were 0.2 μg / kg, the limit of quantification (LOQ) was 0.4 μg / kg, and the relative standard deviations (RSDs) were 1.31% ~ 8.95% The recovery rate reached 74.5% ~ 108.3%. Conclusion The method has high reproducibility and selectivity. It has good application prospects in the determination of malachite green and leucomalachite green in aquatic products.