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虽然ELISA已被广泛应用于测定激素。但是至今临床上还是使用放射免疫法测定血清胰岛素。其主要原因是目前一般使用的ELISA灵敏度难以达到足以测定空腹血清胰岛素的水平。作者通过使用抗胰岛素抗体包被的聚苯乙烯球(直径3.2mm)与50μl的胰岛素或待测血清在一小试管中反应。继之与使用N-(ε-马来酰亚胺已酰)琥珀酰亚胺酯(EMCS)作为双功能试剂制备的抗胰岛素Fab′-辣根过氧化酶(HRP)交联物
Although ELISA has been widely used in the determination of hormones. But so far the clinical use of radioimmunoassay of serum insulin. The main reason is that the current general ELISA sensitivity is difficult to achieve enough to determine fasting serum insulin levels. The authors reacted with 50 μl of insulin or the serum to be tested in a small tube by using polystyrene beads (3.2 mm in diameter) coated with anti-insulin antibody. Followed by an anti-insulin Fab’-horseradish peroxidase (HRP) crosslinker prepared with N- (ε-maleimidocaproyl) succinimidyl ester (EMCS) as a bifunctional reagent